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p90 RSK1 Antibody, HRP

Catalog # Pack Size Price(USD)
ICP9920 100 μg $375.00

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Product Description
Affinity purified rabbit polyclonal anti-p90 RSK1 antibodies were developed with the C-terminal peptide of human RSK1 (p90) and were conjugated to horse-radish peroxidase (HRP) through reductive amination.The conjugate has been used for one-step immunoblotting of crude cell lysates and immunoprecipitated RSK1. The use of HRP conjugates reduce the time of blotting and background, particularly the interference of IgG bands resulting from IP.
 
One step immunoblotting with cross-antibodies of the immunoprecipitated kinases.
A and C: IP with anti-p90 RSK1
B and D: IP with anti-p70 S6K
A and B: IB with anti-p70 S6K HRP conjugate, ICP9917
C and D: IB with anti-RSK1 HRP conjugates, ICP9920
Species
Bovine (brain from Canadian source)
Formulation
250 µg/mL in HRP stabilizing buffer
Immunogen
Human p90 RSK1 C-terminal peptide (C-LAQRRVRKLPSTTL) KLH conjugates
Purification
The antibody was affinity purified with peptide (C-ISKRPEHLRMNL) on agarose.
Conjugation
Reductive amination; HRP/Ab - 2:1
Specificity
The antibody recognizes p90 RSK1 and does not cross react with p70 S6K.
Applications
ELISA; WB
Scientific Description
Rsk1 is a member of a family of 90 kDa ribosomal S6 kinases. It is activated in response to mitogenic stimuli such as extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK, in vitro and in vivo, via phosphorylation and plays an important role in transcription regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.  Expiration date is six months from date of shipping if stored properly.
Product Specific References
1. Curr. Biol. 1999. 9(15): 810-820. doi: 10.1016/S096-9822(99)80364-9