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Anti Interferon Alpha2 HRP 7F4

Catalog # Pack Size Price(USD)
ICP1828 100µg $395.00

Quantity:

Product Description
The anti-Interferon Alpha2 antibody from clone 7F4 was raised with recombinant human interferon Alpha2. The antibody was affinity purified from the cell culture medium using anti-mouse IgG Agarose (ICP9705) affinity chromatography. The purified antibody conjugated to peroxidase (HRP), could be utilized for antigen-capture sandwich ELISA when paired with immobilized antibody from another clone 4F6. The antibody is highly sensitive for ELISA development
      
Detection curve for human interferon a2 (pg/mL). Immobilized antibody: ICP1818 7F4 (1 µg/mL)
Detetion antibody HRP conjugates: ICP1826 4F6 (1 µg/mL)
OD development: TMB at room temperature for 30 min, stop with 1M H2SO4, read at 450nm
Sensitivity: approximately 10-30 pg/mL
Species
Mouse
Clone
7F4
Immunogen
Recombinant human interferon α2
Purification
The antibody was purified from the cell culture         media with anti-mouse IgG specific antibody       Agarose
Conjugation
reductive amination. Ab/HRP ratio=1/2
Formulation
250 μg/mL antibody stored in PBS, 50% glycerol or lyophilized powder
Storage & Stability
Product is stable for 30 days at room temperature. For extended storage, store product at –20ºC. Expiration date is 12 months from date of shipping if properly stored.

Specificity
The antibody recognizes human interferon α2. Cross reactivity to other species not tested
Applications
Interferon-capture sandwich ELISA, by pairing with primary antibody from clone 4F6 (ICP1816)
IP