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P70 S6K Antibody, HRP

Catalog # Pack Size Price(USD)
ICP9917 100 µg $375.00

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Product Description
Affinity purified rabbit polyclonal P70 S6K antibodies were developed using the c-terminal peptide of human S6K (P70) and  conjugated to horse-radish peroxidase (HRP) through reductive amination. This conjugate has been used for one-step immuno-blotting of crude cell lysates and immunoprecipitating P70 S6K. The use of HRP conjugates reduce the time of blotting and background, particularly reducing the interference of IgG bands resulting from IP.
 
Western blot analysis of p70 S6K immunoprecipitated from mouse brain extract (B) and using S6K-HRP as a probe. Immunoprecipitation negative control (A).
Species
Rabbit
Formulation
250 µg/mL in PBS and 50% glycerol
Immunogen
Human p70 S6K C-terminal peptide (C-ISKRPEHLRMNL) KLH conjugates
Purification
The antibody was affinity purified with C-ISKRPEHLRMNL KLH on agarose.
Specificity
Recognizes 70 kDa S6K; does not cross react with RSK1.
Applications
ELISA; WB; IP
Scientific Description
p70 S6K is a protein serine/threonine kinase that exists ubiquitously. [1] p70 S6K affects the activities of the cell cycle. p70 S6K is an important regulator of cell proliferation. [2] p70 S6K activation requires sequential phosphorylation at proline-directed residues in the putative auto-inhibitory pseudo-substrate domain, as well as threonine 389. Activation of p70 S6K requires phosphoinositide 3-kinase (PI3-K)-dependent signal(s).
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
Product Specific References

1. Crit. Rev. Biochem. Mol. Biol. 1994. 29(6): 385-413.
doi:10.3109/10409239409083485

2. J. Biol. Chem. 1996. 271(2): 963-971. doi:10.1074/jbc.271.2.963

3. Mol. Cell Biol. 1999. 19(4): 2921-2928.