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p53 Antibody, HRP

Catalog # Pack Size Price(USD)
ICP7115 100μg $375.00

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Product Description
This antibody was developed and affinity purified using a peptide based on the human p53 sequence (4-40 aa) . The antibody was conjugated to peroxidase (HRP) via reductive amination. It was tested to detect the human p53 specifically in human cell and tissue.  Direct labeling of peroxidase of the primary antibody is believed to avoid the effect of the IgG (50-60 kDa) signal from the use of secondary antibodies.
 
Western blot analysis of p53 in HeLa cell lysates (right) and CHO cell lysates (left) with anti-p53 HRP (ICP7115), 1 µg/mL
Species
Rabbit
Formulation
250 µg/mL in stabilized buffer with 50% glycerol
Immunogen
Human p53 peptide (15 - 40 aa) KLH conjugates
Purification
Peptide-affinity chromatography
Conjugation
Reductive amination; HRP/Ab ratio - 2:1
Specificity
The antibody recognizes human p53 in cell and tissue.
Applications
ELISA (1:2500); WB (1:2500)
Scientific Description
P53 (tumour protein 53 or TP53) regulates the cell cycle by acting as a transcription factor. It suppresses tumours and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman". This is referring to its role in conserving stability by preventing genome mutation. P53 refers to its molecular mass (53 kDa) on SDS-PAGE.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C.Expiration date is six months from date of shipping if stored properly.