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Phospho-Crebtide Antibody

Catalog # Pack Size Price(USD)
ICP0180 100 µg $375.00

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Product Description
Crebtide (KRREILSRRPSYR) is a substrate peptide derived from the protein Creb which is widely utilized as a synthetic substrate for the PKA and PKC kinase families. Affinity purified rabbit polyclonal anti-pCrebtide is developed with phosphorylated Crebtide (KRREILSRRPpSYR) and absorbed with non-phosphorylated Crebtide.  
 
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A: 0.0 ng B: 3 ng C: 6 ng D: 12.5 ng E: 25 ng F: 50 ng
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A:0.0 ng B:4 ng C:15 ng D:60 ng E:250 ng.
Species
Rabbit
Formulation
250 µg/mL in TBS, 0.1% NaN3
Immunogen
Phospho-Crebtide (KRREILSRRPpSYR)-KLH
Purification
The antibody was affinity purified with KRREILSRRPpSYR on agarose followed by affinity desorption with non-phosphorylated Crebtide.
Specificity
The antibody recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay. There are no cross-reactions with the non-phosphorylated Crebtide.
Applications
ELISA; WB; Non-radioactive PKA and PKC kinase assay
Scientific Description
p33 (ING1) is a new growth inhibitor candidate for a tumour suppressor gene. p33 (ING1) cooperates with p53 and down-regulates cell growth by activating transcription from the p21/WAF1 promoter although there is no sequence similarity between p33 (ING1) and p53.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is three years from date of shipping if stored properly.
Product Specific References