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Phospho-Crosstide Antibody

Catalog # Pack Size Price(USD)
ICP0190 100 µg $375.00

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Product Description
Crosstide (PRAATF) is a substrate of the PKB (AKT) and SGK kinase families. Affinity purified rabbit polyclonal anti-pCrosstide antibody is developed with phosphorylated crosstide (PRAApTF) and absorbed with non-phosphorylated crosstide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody has been utilized for non-radioactive PKB and SGK kinase assays.   
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Phospho-crosstide (PRAApTF)-KLH
Purification
The antibody was affinity purified with PRAApTF on agarose followed by affinity absorption with the non-phosphorylated crosstide.
Conjugation
Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin).
Specificity
The antibody only recognizes the phosphorylated crosstide, the product of SGK and PKB in a non-radioactive kinase assay. There are no cross reactions with the non-phosphorylated crosstide.
Applications
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); Non-radioactive AKT and SGK kinase assay
Scientific Description
Crosstide is a synthetic peptide with the sequence (GRPRTSSFAEG). Kinases phosphorylate crosstide to form phospho-crosstide. [1-3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC.  Expiration date is three years from date of shipping if properly stored.

Product Specific References

          1. Nature. 1995. 378: 785-789. doi:10.1038/378785a0                       

        2. Biochem. J. 1996. 315: 709-713.                                             

        3. EMBO J. 1996. 15(23): 6541-6551.