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Phospho-Crosstide Antibody, HRP

Catalog # Pack Size Price(USD)
ICP0192 50 µg $375.00


Product Description
Crosstide (PRAATF) is a substrate of the PKB (AKT) and SGK kinase families. Affinity purified rabbit polyclonal anti-pCrosstide antibody is developed with phosphorylated crosstide (PRAApTF) and absorbed with non-phosphorylated crosstide. The purified antibody was conjugated to peroxidase (HRP) via reductive amination. The antibody has been utilized for non-radioactive PKB and SGK kinase assays.   
250 µg/mL in PBS, pH 7, 50% glycerol
Phospho-crosstide (PRAApTF)-KLH
The antibody was affinity purified with PRAApTF on agarose followed by affinity absorption with the non-phosphorylated crosstide.
Reductive amination; HRP/Ab ratio - >2
The antibody only recognizes the phosphorylated crosstide, the product of SGK and PKB in a non-radioactive kinase assay. There are no cross reactions with the non-phosphorylated crosstide.
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
Scientific Description
Crosstide is a synthetic peptide with the sequence (GRPRTSSFAEG). Kinases phosphorylate crosstide to form phospho-crosstide. [1-3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.
Product Specific References

        1. Nature. 1995. 378: 785-789. doi:10.1038/378785a0                       

        2. Biochem. J. 1996. 315: 709-713.                                             

        3. EMBO J. 1996. 15(23): 6541-6551.