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AFB1 (Aflatoxin B1) HRP

Catalog # Pack Size Price(USD)
ICP1206 100 μg $350.00

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Product Description
This product is Aflatoxin B1 to Horse radish peroxidase (HRP). It is useful as an antigen-labeled peroxidase paired with the anti-Aflatoxin antibody in a direct ELISA.
 
 Structure of the AFB1-HRP Conjugates
Formulation
500 µg/mL in 50% glycerol with 1% BSA or lyophilized powder.
Conjugation
Afaltoxin B1 methylcarboxyloxime linked to HRP via amine acylation. AFB1/HRP molar ratio:1:1.
ELISA Titer
OD50 ELISA titer for  AFB1-HRP is approximately 0.1 µg/mL in plate immobilized with 0.5 µg/mL of monoclonal anti-AFB1.  OD signal was 95% inhibited by 5 ng/mL of free AFB1 from 0.1-1 ng/mL.
Applications
ELISA: suggested use concentration: 0.1-0.5  ng/mL. Gold colloid: to be optimized by users.
Storage & Stability
Product is stable for at least 30 days at room temperatures and for one year  when stored at –20ºC. Expiration date is one year from date of shipping if properly stored.