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Chloramphenicol Antibody

Catalog # Pack Size Price(USD)
CN1025 100 μg $295.00

Quantity:

Product Description
This antibody was developed using chloramphenicol-KLH conjugates as the immunogen and purified with chloramphenicol-agarose  affinity chromatography. The antibody could be utilized for isolation, detection and quantization of chloramphenicol.
 
Direct competitive inhibition ELISA using immobilized anti-chloramphenicol (3 µg/mL), and chloramphenicol-labelled HRP (100 ng/mL). 50% inhibition occurred at 50 ng/mL of free chloramphenicol under non-optimized conditions.
Species
Rabbit
Formulation
250 µg/mL in PBS with 50% glycerol
Immunogen
Chloramphenicol-KLH conjugates
Purification
Antigen-specific affinity chromatography
Specificity
The antibody recognizes free chloramphenicol.
Applications
ELISA; Affinity chromatography
Scientific Description
Chloramphenicol is a bacteriostatic antimicrobial agent originally derived from the bacterium Streptomyces venezuelae, isolated by David Gottlieb, and introduced into clinical practice in 1949. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms and is still very widely used in low income countries because it is exceedingly inexpensive. Detection and quantization of chloramphenicol in food (feed) is becoming common due to the issue of antibiotic abuse.  
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at –20ºC. Expiration date is one year from date of shipping if properly stored.