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Human IgG Antibody, Agarose

Catalog # Pack Size Price(USD)
ICP9715 20mg $295.00

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Product Description
Affinity purified goat anti-human IgG antibodies were developed using purified human IgG as the immunogen. The antibodies are covalently linked to agarose beads and useful for isolation and purification of human immunoglobulin G.
 
Elution profile of human IgG from affinity chromatography based on the anti-human IgG agarose.
      i.   Serum loading:  250 µL, diluted with distilled water to 1 mL
     ii.   Anti-human IgG Agarose: 2 mL
    iii.   Single Pass: 10 min
    iv.   IgG Binding: >4 mg/mL; remove all the IgG from 250 µL of human serum
SDS Profile: MW markers (1); Human IgG Std (2); Human serum albumin Std (3); Elution fraction 1-5 (3, 4, 5, 6, 7); Original human serum (8) and human serum flow through (9).
 
Species
Goat
Formulation
2 mL in 4 mL slurry of 50% glycerol with 0.01% NaN3
Immunogen
Pure human IgG (whole molecule)
Purification
Affinity purified with human IgG immobilized on agarose and absorbed with BSA and mouse antibodies.
Specificity
Human IgG; does not cross react with human serum albumin or mouse IgG
Antibody Immoblilzed
>30 mg/mL antigen-specific affinity-purified anti-human IgG
Binding Capacity
>10 mg human IgG/mL
Applications
Human IgG removal, isolation and purification
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.