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Mouse IgG Antibody, Agarose

Catalog # Pack Size Price(USD)
ICP1678 30 mg $350.00

Quantity:

 

Product Description

 

 

 

 

 

 

Monoclonal antibody production using cell culture systems sometime requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from mouse serum, ascites and the supernatant of the hybridoma culture media, without pulling-down bovine IgG. This anti-mouse IgG agarose was prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the Agarose beads via a highly stable thiol-ether bond. This product has been successfully used to replace Protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity > 10 mg/mL. 

 

 
Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.

Fraction 1: 0.8 mg/mL

Fraction 2: 2.5 mg/mL

IgG Std: 0.31 - 5 mg/mL

 

Species

 

 Goat

Formulation

 

1 mL in 2 mL PBS with 70% glycerol

Ligand Immobilized

 

30-40 mg antigen specific affinity-purified anti-mouse IgG/mL

Applications
Affinity chromatography; Immunoprecipitation

Binding Capacity

 

>10 mg/mL of mouse IgG

Storage & Stability 
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.