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Human IgG Antibody, Agarose

Catalog # Pack Size Price(USD)
ICP1648 30 mg $350.00

Quantity:

 
Product Description

Affinity purified rabbit anti-human IgG antibodies are developed using purified human IgG as immunogen. The antibodies are covalently linked to agarose beads, useful for isolation and purification of human immunoglobulin G.

 
 Sample Data

Elution profile of human IgG from affinity chromatography based on the anti-human IgG agarose

i. Serum loading:  250 µL, diluted with distilled water to 1 mL

ii. Anti-human IgG Agarose: 2 mL

iii. Single Pass: 10 min

iv. IgG Binding: >4 mg/mL; remove all the IgG from 250 µL of human serum

SDS Profile: MW markers (1); Human IgG Std (2); Human serum albumin Std (3); Elution fraction 1-5 (3, 4, 5, 6, 7); Original human serum (8) and human serum flow through (9).

 
Species

Goat

 

Formulation

1 mL in 2 mL slurry of 50% glycerol with 0.01% NaN3

 

 

Immunogen

 

 Pure human IgG (whole molecules)

Purification

Affinity purified with  human IgG immobilized on agarose and absorbed with BSA and mouse antibodies.

 

Specificity 

Human IgG; does not cross react with human serum albumin and mouse IgG

 

Applications

Human IgG removal, isolation and purification

 

Antibody Immobilized >30 mg/mL antigen-specific affinity-purified anti-human IgG
Storage & Stability 

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.