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Biotin Antibody Agarose

Catalog # Pack Size Price(USD)
ICP0615 10 mg $695.00

Quantity:

Product Description
The biotin antibody is developed using biotin-KLH conjugates and purified with biotin-agarose affinity chromatography. The purified biotin antibody was chemically linked to beaded agarose through a stable covalent linkage. The immobilized biotin antibody on beaded agarose could be utilized for reversible immunoprecipitation and affinity purification of both biotinylated antibodies and biotinylated proteins. This product has been applied to the MS-based proteomics experiments as per paper (Udeshi et al in Nature Methods Vol.14 pages: 1167-1170 (2017)).
Formulation
1 mL beads in 2 mL slurry with glycerol
Antibody Immobilized
10 mg/mL agarose
 
Binding       Capacity
> 10 mg/mL of biotinylated BSA
Applications
IP; Immuno-affinity chromatography; 
Scientific Description
Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding of the biotinylated species could be competitively eluted with free biotin.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Do not aliquot and shake throughly before use. Expiration date is three years from date of shipping if properly stored. 
Product Specific References 
  1. 1. Nature Methods. 2017. 14 (12): 1167-1170. doi: 10.2016/j.bbrc.2014.07.110.