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Mouse IgG Antibody, Agarose

Catalog # Pack Size Price(USD)
ICP9705 40 mg $350.00

Quantity:

Product Description
Monoclonal antibody production using cell culture systems somtimes requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from both mouse serum, ascites and the supernatant, of the cell culture media, without pulling-down boving IgG. This anti-mouse IgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product as been successfully used to replace protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity > 10 mg.mL.
Formulation
1 mL beads in 2 mL glycerol
Ligand Immobilized
40 mg specific affinity-purified anti-mouse IgG/mL
Binding Capacity
>10 mg/mL of mouse IgG
Applications
Affinity chromatography ; Immunoprecipitation
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is three years from date of shipping if stored properly.
 
Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.
Fraction 1: 0.8 mg/mL
Fraction 2: 2.5 mg/mL
IgG Std: 0.31 - 5 mg/mL