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HA Antibody, HRP

Catalog # Pack Size Price(USD)
ICP0751 100 μg $295.00

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Product Description
This high affinity anti-HA antibody is developed using the HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. This antibody is conjugated to horse radish peroxidase (HRP) through reductive amination. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates.
 
Western blot analysis of the HA-tag protein (50 kDa) expressed cell lysates with HA antibody HRP conjugates (ICP0751)              1:  Marker                                                            2: 25  µL/lane                                                      3: 15  µL/lane                                                      4: 5 µL/lane                                                          5: 2 µL/lane
               A         B        C        
Western blot analysis of the positive cell lysates with expression of HA-tag protein.  
 A:  Marker                                                            B: 1  µL/lane                                                      C: 10  µL/lane 
Species
Rabbit
Formulation
250 µg/mL antibody in peroxidase buffer
Immunogen
Human influenza virus hemaglutinin (HA) peptide, (YPYDVPDYA) KLH conjugates
Purification
Antigen-specific affinity chromatography
Specificity
The antibody recognizes proteins fused with the (HA) peptide, YPYDVPDYA.
Conjugation
Reductive amination; HRP/Ab molar ratio - 10:1
Applications
ELISA; WB; IP
Scientific Description
Human influenza virus hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of HA-fusion proteins.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.