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Acetyl Lysine Antibody, FITC

Catalog # Pack Size Price(USD)
ICP0385 100 µg $375.00

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Product Description
The affinity purified rabbit polyclonal anti-acetylated lysine antibody was developed using a unique technique that utilizes acetylated KLH as the immunogen and acetylated lysine-specific antibodies. It was specifically purified with immobilized acetylated lysine on agarose. The purified antibody is conjugated to fluorescein isothiocyanate.
 
Immunoflourescent staining of the TSA-treated and non-treated melanoma cells.
Species
Rabbit
Formulation
250 µg/mL antibody in PBS, pH 7, with 50% glycerol
Immunogen
Acetylated KLH conjugates
Purification
The antibody was purified on acetyl-lysine agarose.
Conjugation
FITC/Ab ratio - 10:1, followed by G25 filtration
Applications
For direct immunofluorescence experiments
Scientific Description
Acetylation of lysine is an important, reversible modification. The activities of some proteins are controlled by acetylation of lysine. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysine residues. Histone deacetylases (HDACs) remove the acetyl group from the same residue. [1] Acetylation/deacetylation of histones results in cell signalling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired (7). Numerous anti-cancer drugs target HDACs. [3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C.  Expiration date is three years from date of shipping if stored properly.

Product Specific References

  1.  1. Curr. Opin. Chem. Biol. 1997. 1(3): 300-308. doi:10.1016/S1367-5931(97)80066-X.
  2.  2. Curr. Cancer Drug Targets. 2004. 4(2): 205-218.  doi:10.2174/1568009043481560.