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Phosphoserine Antibody

Catalog # Pack Size Price(USD)
ICP9806 100 µg $325.00


Product Description
Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source.
Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):
Lane1-2:  Kinase untreated
Lane 3-6: kinase treated with phosphatase PP1G
Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates.
A: Control
B-D: Treatment with increased UV dose
E: Phosphatase inhibitor treatment
High affinity anti-phospho-serine antibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin and α-casein) but not non-phospho proteins (ovalbumin, BSA, β-lactoglobulin, trypsinogen) 

250 µg/mL in PBS, 50% glycerol



Phospho-serine KLH and phosvitin mixture



Affinity purified with phospho-tyrosine on agarose



The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.



Human breast cancer tissue and mouse cell



ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50)


Scientific Description

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.


Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.


Product Specific References

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  6. 6. JAHA. 2018; 7(1) doi: 10.1161/JAHA.117.007185
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  8. 8. JAHA. 2018. 7(1). doi: 10.1161/JAHA.117.007185
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  11. 11. American Journal of Human Genetics. 2018. 102(4): 649-657. doi: 10.1016/j.ajhg.2018.02.015