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Phospho-Serine Antibody
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Product Description
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Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source.
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 Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):
Lane1-2: Kinase untreated
Lane 3-6: kinase treated with phosphatase PP1G
 A: Control
B-D: Treatment with increased UV dose
E: Phosphatase inhibitor treatment
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Species
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Rabbit | ||
| Formulation | 250 µg/mL in PBS, 50% glycerol | ||
| Immunogen | Phospho-serine KLH and phosvitin mixture | ||
| Purification | Affinity purified with phospho-tyrosine on agarose | ||
| Specificity | The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine. | ||
| Tested | Human breast cancer tissue and mouse cell | ||
| Applications | ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50) | ||
| Scientific Description | Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes. | ||
| Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. | ||
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Product Specific References |
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