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Phosphoserine Antibody

Catalog # Pack Size Price(USD)
ICP9806 100 µg $325.00

Quantity:

Product Description
Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source.
 
 
Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):
Lane1-2:  Kinase untreated
Lane 3-6: kinase treated with phosphatase PP1G
Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates.
A: Control
B-D: Treatment with increased UV dose
E: Phosphatase inhibitor treatment
High affinity anti-phospho-serine antibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin and α-casein) but not non-phospho proteins (ovalbumin, BSA, β-lactoglobulin, trypsinogen) 
Species
Rabbit
Formulation

250 µg/mL in PBS, 50% glycerol

 

Immunogen

Phospho-serine KLH and phosvitin mixture

 

Purification

Affinity purified with phospho-tyrosine on agarose

 

Specificity

The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.

 

Tested

Human breast cancer tissue and mouse cell

 

Applications

ELISA (1:250); WB (1:250); IP (10 µg/mg crude protein); IHC (1:50)

 

Scientific Description

Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes.

 

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

 

Product Specific References

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  2. 2. Nat. Commun. 2016. 7: 11151. doi:10.1038/ncomms11151
  3. 3. Nature Communications. 2016. 7:11151: 1-15. doi:I: 10.1038/ncomms11151
  4. 4. Front Immunol. 2017; 8: 352. doi: 10.3389/fimmu.2017.00352.
  5. 5. Front Immunol. 2017; 8: 189. doi: 10.3389/fimmu.2017.00189.
  6. 6. JAHA. 2018; 7 (1) doi: 10.1161/JAHA.117.007185
  7. 7. AJHG. 2018. 102 (4): 649-657. doi: 10.1016/j.ajhg.2018.02.015
  8. 8. JAHA. 2018. 7 (1). doi: 10.1161/JAHA.117.007185
  9. 9. Cellular Physiology and Biochemisty. 2018. 46 (1). doi: 10.1159/000488418