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Phospho-Threonine Antibody

Catalog # Pack Size Price(USD)
ICP9807 100 µg $295.00

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Product Description
Affinity purified rabbit polyclonal phospho-threonine antibody (anti-pT) was developed using a phospho-threonine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-threonine specific antibody was affinity purified with phospho-threonine immobilized on agarose.
 
Detection of a phosphorylated protein kinase with anti-phosphothreonine (ICP9807)
Lane1-2:  Untreated kinase
Lane 3-4: Kinase treated with  phosphatase PP1G
Western blot analysis of mouse extract spleen with ICP9807
A: Extract of spleen from mouse treated  with sodium vanadate
B: Extract of spleen from control mouse
Species
Rabbit
Formulation
250 µg/mL in PBS, pH 7, with 50% glycerol
Immunogen
Phospho-threonine KLH conjugates and phosvitin mixture
Purification
The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
Specificity
The antibody recognizes proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phospho-tyrosine.
Species Tested
Human and mouse
Applications
ELISA; WB; IP
Scientific Description
Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phospho-threonine which enters various biosynthetic pathways in the body.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

  1. 1. Betts, M. J. and Russell, R. B. (2003) Amino Acid Properties and Consequences of Substitutions, in Bioinformatics for Geneticists (eds M. R. Barnes and I. C. Gray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867302.ch14