Home >PTM Proteomics > Phosphorylation

Phospho-Threonine Antibody, Biotin

Catalog # Pack Size Price(USD)
ICP9820 100 µg $375.00

Quantity:

Product Description
Affinity purified rabbit anti-phospho-threonine antibodies are conjugated to d-biotin via N-acetylation. The conjugates are useful for detection, identification and quantization of threonine-phosphorylated proteins and peptides.
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Phospho-threonine-KLH conjugates and phosvitin
Purification
The antibody was purified on phospho-threonine agarose.
Conjugation
Acetylation of the primary amine with the active ester of biotin (NHS-activated d-biotin)
Specificity
The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine.
Applications
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
Scientific Description
Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate threonine to form phospho-threonine which is considered a key step in the regulation of enzymatic activities such as signal transduction.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

  1. 1. Betts, M. J. and Russell, R. B. (2003) Amino Acid Properties and Consequences of Substitutions, in Bioinformatics for Geneticists (eds M. R. Barnes and I. C. Gray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867302.ch14