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Phospho-Threonine Antibody, HRP

Catalog # Pack Size Price(USD)
ICP9821 100 µg $375.00

Quantity:

Product Description
The affinity purified rabbit anti-phospho-threonine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of threonine-phosphorylated proteins and peptides.
Species
Rabbit
Formulation
250 µg/mL in PBS, pH 7, with 50% glycerol
Immunogen
Phospho-threonine-KLH conjugates and phosvitin
Purification
The antibody was affinity purified with phospho-threonine on agarose followed by affinity absorption with threonine on agarose.
Specificity
The antibody is pan-specific against phospho-threonine. It cross reacts with phospho-serine slightly but does not cross-react with phospho-tyrosine.
Applications
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
Scientific Description
Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to form phospho-threonine, which enters various biosynthetic pathways in the body.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

  1. 1. Betts, M. J. and Russell, R. B. (2003) Amino Acid Properties and Consequences of Substitutions, in Bioinformatics for Geneticists (eds M. R. Barnes and I. C. Gray), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/0470867302.ch14