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Phospho-Tyrosine Antibody, Biotin

Catalog # Pack Size Price(USD)
ICP9901 100 µg $375.00

Quantity:

Product Description
The affinity purified rabbit anti-phospho-tyrosine antibody was conjugated to d-biotin via N-acetylation. The conjugates are useful for the detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. These conjugates have been utilized for non-radioactive tyrosine kinase assays.
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol, 0.01% NaN3
Immunogen
Iodoacetylphosphotyamine-KLH conjugates
Purification
The antibody was affinity purified with phospho-tyrosine immobilized on agarose.
Specificity
The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
Applications
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL); IP (5 µg/mg protein); IHC (10 µg/mL)
Scientific Description
Threonine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine, which is considered a key step in the regulation of enzymatic activities such as signal transduction.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is one year from date of shipping if properly stored.

Product Specific References

  1. 1. J. Bio. Chem. 2006. 281: 37877-37887doi:10.1074/jbc.M609398200