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Phospho-Tyrosine Antibody, HRP

Catalog # Pack Size Price(USD)
ICP9902 100 µg $375.00

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Product Description
The affinity purified rabbit polyclonal anti-phospho-tyrosine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides.
 
Immunoprecipitated samples with anti-phospho-tyrosine agarose (ICP9804)
Left: Mouse spleen lysate treated with complete adjuvant   Right: Mouse spleen lysate without any treatment   Detection: Phospho-tyrosine antibody, HRP (ICP9902)
Species
Rabbit
Formulation
250 µg/mL in HRP stabilizing buffer with 50% glycerol
Immunogen
Iodoacetylphosphotyramine-KLH conjugates
Purification
The antibody was affinity purified with phospho-tyrosine immobilized on agarose, followed by immune affinity absorption with tyrosine on agarose.
Conjugation
Reductive amination; HRP/Ab ratio - 10:1
Specificity
The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
Applications
ELISA (1:1000); WB (1:1000)
Scientific Description
Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

  1. 1. J. Bio. Chem. 2006. 281: 37877-37887. doi:10.1074/jbc.M609398200