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Methyl (mono) Arginine Antibody

Catalog # Pack Size Price(USD)
ICP0801 100 μg $325.00


Product Description
This antibody is raised from rabbits that were immunized with NG- mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are useful for detection, isolation and localization of proteins with Nγ-methylated arginine residues.

Competitive ELISA: inhibition of the binding mMeR-HRP to anti-mMeR antibody immobilized (1µg/mL) by free mMeR (IC50=40-50 ng/mL)



Western blot of human MMRU cells with ICP0801 (A,B) plus competitive inhibition with free methyl arginine (C,D)

Fluorescent Staining of MMRU cells

250 μg/mL in PBS, 50% glycerol
NG-mono-methyl arginine-KLH conjugates
Purified by antigen-specific affinity chromatography 

The antibody recognizes free mono-methyl arginine and proteins with methylated arginine residues.  It does not cross react with either asymmetric or symmetric dimethyl arginine.

Species Tested
Pan-specific regardless of species.
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at –20ºC. Expiration date is three years from date of shipping if properly stored.

Product Specific References


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