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Methylated Lysine Antibody, Biotin

Catalog # Pack Size Price(USD)
ICP0503 100 μg $375.00

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Product Description
The affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to d-biotin via acetylation. Direct labelling of the primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody conjugates.
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Methylated KLH conjugates
Purification
Affinity purified with methyl-lysine and di-methyl-lysine on agarose
Conjugation
Acetylation with NHS-biotin; Biotin/Ab molar ratio - 10:1
Specificity
The antibody recognizes anti-MeK (biotin conjugate) and recognizes proteins methylated on their lysine residues (mono and di-methyl lysine). It does not cross react with acetylated proteins.
Applications
ELISA (0.25 - 1.0 µg/mL); WB (0.25 - 1.0 µg/mL)
Scientific Description
MeK is a conserved post-translational modification which is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome B, chromosomal proteins, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC.  Expiration date is one year from date of shipping if properly stored.
Product Specific References

        1. J. Biol. Chem. 2003. 278: 18346-18352. doi.10.1074/jbc.M300890200

        2J. Biol. Chem. 2002. 277: 34655-34657. doi:10.1074/jbc.C200433200

        3. J. Biol. Chem. 2002. 277: 11621-11624. doi:10.1074/jbc.C200045200