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Dimethyl Arginine Antibody

Catalog # Pack Size Price(USD)
ICP0810 100 μg $325.00

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Product Description
This antibody was raised from rabbits that were immunized with NG,NG-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the NG,NG-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues.
 
Western blot of human MMRU cells with ICP0810 (A,B) plus competitive inhibition with free dimethyl arginine (C,D)
 
Antigen Structure
 
[Exposure: 10 Milliseconds]
IF Staining of MMRU cells
 
Species
Rabbit
Formulation
250μg/mL antibody stored in PBS, 50% glycerol
Immunogen
NG,NG-dimethyl arginine-KLH conjugates
Purification
Antigen-specific immunoaffinity purification with NG,NG-dimethyl arginine and adsorbed with NG-monomethyl arginine
Specificity
The antibody recognizes proteins with dimethylated arginine residues.  It does not cross-react with acetylated proteins or monomethylated proteins.
Species Tested 
Human, mouse and pig
Applications
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is three years from date of shipping if properly stored.

Product Specific References

 
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4. Proteomics2013. 13 (7): 1059-1064. doi: 10.1002/pmic.201200477
5. Proteomics 2015. 15 (18): 3209-3218. doi: 10.1002/pmic.201500075
6. J. Neurosci. 2015. 35 (37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015