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NG-monomethyl Arginine Antibody (anti-mMeR), HRP

Catalog # Pack Size Price(USD)
ICP0802 100µg $375.00

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Product Description
This antibody was raised from rabbits that were immunized with NG-mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are conjugated to peroxidase (HRP) and are useful for detection, isolation and localization of proteins with NG-methylated arginine residues.
 
Antigen Structure
Species
Rabbit
Formulation
250 µg/mL in stabilizing buffer with 50% glycerol
Immunogen
N5-mono-methyl arginine-KLH conjugates
Purification
Antigen-specific affinity chromatography followed by absorption with dimethyl arginine
Conjugation
Reductive amination; HRP/Ab ratio: 2:1
Specificity
The antibody recognizes proteins with methylated arginine residues. It does not cross react with acetylated proteins or mono- and dimethylated proteins.
Species Tested 
Human, mouse and pig
Applications
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes including protein trafficking, signal transduction and transcriptional regulation.  
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
Product Specific References
1. Mol Microbiol. 2007. 65: 590-606. doi:10.1111/j.1365-2958.2007.05831.x