Home >PTM Proteomics > Protein Methylation

Methylated Lysine Antibody, FITC

Catalog # Pack Size Price(USD)
ICP0505 100 μg $375.00

Quantity:

Product Description
Affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a methylated protein as the immunogen. The methyllysine specific antibodies are affinity purified with N-e-mono-methyllysine and di-methyllysine on agarose as the affinity matrix. The purified antibody was conjugated to fluorescein. The conjugated antibody was tested to localize methylated proteins.
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Methylated KLH conjugates
Purification
N-e-mono-methyllysine and di-methyllysine on agarose
Specificity
Recognizes proteins methylated on lysine residues (mono and di-methyllysine). It does not cross react with acetylated proteins. The cross reactivity to tri-methyllysine has not been tested.
Conjugation
With fluorescein isothiocyanate (FITC)
FITC/Ab molar ratio - 10:1
Applications
IF (1:50 - 1:100)
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC.  Expiration date is one year from date of shipping if properly stored.