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NG,NG-dimethyl Arginine Antibody (anti-dMeR), HRP

Catalog # Pack Size Price(USD)
ICP0812 100 µg $375.00

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Product Description
This antibody is raised from rabbits that were immunized with NG,NG-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the NG,NG-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues.
 
 
Antigen Structure
Species
Rabbit
Formulation
250 µg/mL in stabilizing buffer with 50% glycerol
Immunogen
N5,NG-dimethyl arginine-KLH conjugates
Conjugation
Aldehyde-activated HRP via reductive amination
HRP/Ab ratio: 3:1
Specificity
The antibody recognizes proteins with dimethylated arginine residues. It does not cross react with acetylated proteins or mono-methylated proteins.
Species Tested
Human, mouse and pig
Applications
ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL); IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes including protein trafficking, signal transduction and transcriptional regulation.  
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly.
Product Specific References
1. Mol Microbiol. 2007. 65: 590-606. doi:10.1111/j.1365-2958.2007.05831.x