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Di-methyl (ε-N) Lysine Antibody, HRP

Catalog # Pack Size Price(USD)
ICP0702 100 μg $395.00

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Product Description
The affinity purified rabbit polyclonal anti-Di-methylated lysine antibodies are developed using a technique unique to ImmuneChem. The Di-methylated lysine antibodies are affinity purified using N-Di-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibodies are conjugated to horse radish peroxidase (HRP). The antibody-HRP conjugates could be utilized for detection, quantization and localization of proteins with Di-methylated lysine residues.
 
 
Western blot analysis of the di-methylated proteins from swine spleen cell lysate with (B) and without (A) the presence of methylated BSA, using anti-di-methyl lysine antibody HRP conjugates (anti-MeK HRP, ICP0702)
Species
Rabbit
Formulation
250 µg/mL in Tris-phosphate buffer with 1% BSA and 50% glycerol
Immunogen
Di-methylated KLH conjugates
Purification
The antibody is  immunoaffinity purified with epsilon N-Di-methyl lysine on agarose.
Conjugation
Reductive amination with antibody/HRP molar ratio = 1:1
Specificity
The antibody recognizes proteins or peptides with di-methyl lysine residues. There is no cross reaction with acetylated proteins. The cross reactivity with mono-methyl lysine has not been tested.
Applications
ELISA, WB
Scientific Description
Protein lysine methylation (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example, calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that di-methylation of lysine plays an important role in gene silencing.[1]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. Do not aliquot. Shake thoroughly before use.

Product Specific References

  1.  1. Mol. BioSyst. 2013. 9: 2231-2247. doi: 10.1039/C3MB00009E