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Dimethyl (sym) Arginine Antibody

Catalog # Pack Size Price(USD)
ICP0811 100 μg $325.00

Quantity:

 

Product Description

 

This antibody is raised from rabbits that were immunized with NG,NG′-Dimethyl-L-arginine-KLH conjugates and specifically isolated and affinity purified using the NG,NG′-Dimethyl-L-arginine antigen Agarose column. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues.

 
 
 




















 
Western blot of human MMRU cells with Diemthyl (sym) Arginine antibodies (ICP0811) and Actin antibody

Immunogen Structure

 
 

 

 
 
 

 


 

Species
Rabbit
Formulation

250 µg/mL in PBS, 50% glycerol   
Immunogen

NG,NG′-Dimethyl-L-arginine-KLH conjugates
Purification

Antigen-specific immunoaffinity purification with NG,NG′-Dimethyl-L-arginine and absorbed with NG-mono-methyl arginine.
Specificity

The antibody recognizes proteins with dimethylated arginine residues. It does not cross react with acetylated proteins or mono-methylated proteins.
Applications
ELISA (1:2000), WB (1:1000), IP (5 µg/mg protein sample), IF (5 µg/mL), IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.  
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is three years from date of shipping if stored properly.

Product Specific References

  1. 1. J. Neurosci. 2015. 35(37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015