Home >PTM Proteomics > Protein Methylation

NG-monomethyl Arginine, HRP

Catalog # Pack Size Price(USD)
ICP0868 200 µg $350.00

Quantity:

 

Product Description

NG-monomethyl Arginine (mMeR) peroxidase (HRP) conjugates were developed by covalently linking mMeR to HRP through reductive amination method. mMeR-labelled HRP could be captured specifically by anti-mMeR antibody (ICP0801), as well could be utilized for direct competitive ELISA for the detection and quantization of mMeR.

 

 

 

Direct competitive inhibition ELISA using immobilized anti-mMeR (1 µg/mL), and mMeR-labelled HRP (100 ng/mL), 50% inhibition occurred at approximately 20-40 ng/mL of free mMeR in non-optimized conditions.

 

Structure of the mMeR HRP Conjugate

Formulation

0.5 mg/mL in peroxidase stabilizing buffer or lyophilized.

 

Conjugation

Reductive amination. mMeR/HRP molar ratio, approximately 3:1.

 

Applications

ELISA

 

Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Avoid cycles of freezing and thawing. Expiration date is three years from date of shipping if properly stored.