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Mono-Methyl (ε-N) Lysine Antibody

Catalog # Pack Size Price(USD)
ICP0518 100 μg $325.00
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Product Description

The affinity purified rabbit polyclonal anti-Mono-methylated lysine antibody was developed using a technique unique to ImmuneChem. The Mono-methylated lysine antibodies are affinity purified using N-Mono-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with Mono-methylated lysine residues.



Western blot analysis of the Mono-methylated histone from melanoma cells with anti-mono-Methylated lysine (anti-mMeK (ICP0518). Histones were isolated with cold 0.1M HCl extraction.

A: Blot with 0.5 µg/mL of anti-mMeK (ICP0518) in PBSt + methylated BSA (ICP0504) 100 µg/mL

B: Blot with 0.5 µg/mL of anti-mMeK (ICP0518) in PBSt







250 µg/mL in PBS, 50% glycerol or lyophilized powder.




Mono-methylated KLH conjugates



The antibody recognizes proteins or peptides Mono-methylated on the lysine residues. The antibody is slightly (30%) cross-reacted with dimethyllysine, not cross-reacted with trimethylysine and acetyllysine.





The antibody was immunoaffinity purified with epsilon N-mono-methyl lysine on agarose.






ELISA: 0.25-0.5 ug/mL

WB: 0.25-0.5 ug/mL

IHC: 2-5 ug/mL

IP: 10-20 ug/mg crude protein





Scientific Description

MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that Mono-methylation of lysine plays an important role in gene silencing.



Storage & Stability

Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is three years from date of shipping if stored properly.