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Methylated Lysine Antibody, HRP

Catalog # Pack Size Price(USD)
ICP0502 100 μg $375.00

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Product Description
The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labelling of primary anti-MeK will avoid the use of secondary antibodies therefore eliminating the interference of the secondary antibody-conjugates. The antibodies could be utilized for detection, quantization and isolation of proteins with MeK residues.
 
Western blot analysis of the methylated protein pattern in whole HeLa cells with ICP0502.
A. Signal of methylated proteins
B. Signal of the methylated proteins was competitively suppressed by 50 µg/mL of methylated BSA
C. Signal of the methylated proteins was competitively suppressed by 10 µg/mL of methylated BSA
 
Species
Rabbit
Formulation
500 µg/mL in HRP stabilizing buffer with 50% glycerol
Immunogen
Methylated KLH conjugates
Purification
Methyl-lysine and di-methyl lysine on agarose
Conjugation
Reductive amination; HRP/Ab molar ratio - 10:1
Specificity
Anti-MeK peroxidase conjugates recognize proteins methylated on their lysine residues (mono and di-methyllysine). They do not cross react with acetylated proteins.
Applications
ELISA; WB
Scientific Description
MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
Product Specific References

1. J. Biol. Chem. 2003. 278: 18346-18352. doi.10.1074/jbc.M300890200

2J. Biol. Chem. 2002. 277: 34655-34657. doi:10.1074/jbc.C200433200

3. J. Biol. Chem. 2002. 277: 11621-11624. doi:10.1074/jbc.C200045200