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Trimethyl Lysine Antibody

Catalog # Pack Size Price(USD)
ICP0601 100 μg $350.00


Product Description
This rabbit polyclonal antibody is specifically isolated and affinity purified from the anti-serum using chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues.
Immunohistochemistry of the human tissue with ICP0601
Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with ICP0601.                                            A. Primary antibody                      B. Primary antibody plus 50mg/mL of methylated protein BSA (ICP0504)
250 µg/mL in PBS, 50% glycerol
Methylated KLH conjugates
N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose
FITC/Ab ratio - 10:1, followed by G25 filtration
Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.
Species   Tested
Human, rat, mouse, bovine and pig
ELISA (1:2000); WB (1:2000); IP (5 µg/mg protein sample); IF (5 µg/mL or 1:100)
Scientific Description
In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe2+, and oxygen.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Expiration date is one year from date of shipping if stored properly.

Product Specific References

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