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Trimethyl Lysine Antibody

Catalog # Pack Size Price(USD)
ICP0601 100 μg $325.00

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Product Description
This rabbit polyclonal antibody is specifically isolated and affinity purified from the anti-serum using chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues.
 
Immunohistochemistry of the human tissue with ICP0601
 
Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with ICP0601.                                            A. Primary antibody                      B. Primary antibody plus 50mg/mL of methylated protein BSA (ICP0504)
Species
Rabbit
Formulation
250 µg/mL in PBS, 50% glycerol
Immunogen
Methylated KLH conjugates
Purification
N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose
Conjugation
FITC/Ab ratio - 10:1, followed by G25 filtration
Specificity
Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.
Species   Tested
Human, rat, mouse, bovine and pig
Applications
ELISA (1:2000); WB (1:2000); IP (5 µg/mg protein sample); IF (5 µg/mL or 1:100)
Scientific Description
In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe2+, and oxygen.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C. Expiration date is three years from date of shipping if stored properly.

Product Specific References

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8. Sci Rep. 2016; 6: 35432. doi: 10.1038/srep35432

9. Proteomics 2015, 15, 2166–2176. doi: 10.1074/jbc.M114.605527.

10. Applied and Environmental Microbiology. 2017, Volume 83, Issue 11. doi: 10.1128/AEM.00800-17

11. THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 289, NO. 44, pp. 30511–   30524, October 31, 2014. doi: 10.1002/pmic.201400521.