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Tri-methyl Lysine Antibody (pan specific), HRP

Catalog # Pack Size Price(USD)
ICP0602 100 μg $395.00

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Product Description
This rabbit polyclonal antibody is isolated and affinity purified from the anti-serum against chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The purified antibody is conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labelling of primary antibodies will avoid the use of secondary antibodies therefore eliminating the effects of the secondary antibody-conjugates. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues.
 
Western blot analysis of trimethylated protein from human melanoma cells (MMRU) with ICP0602
A: Competitive inhibition with N-(epsilon) Trimethyl-lysine (50µg/mL)
B: Without competitive inhibition with N-(epsilon) trimethyl lysine
 
Western blot analysis of trimethylated protein from the acid extract of calf spleen with ICP0602 
C: Competitive inhibition with N-(epsilon) trimethyl lysine (50µg/mL)
D: Without competitive inhibition with N-(epsilon) trimethyl lysine
Formulation
500 µg/mL in HRP stabilizing buffer with 50% glycerol
Immunogen
Methylated KLH conjugates
Purification
N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose
Conjugation
Reductive amination; HRP/Ab molar ratio - 2:1
Specificity
Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.
Species Tested
Human, rat, mouse, bovine and pig
Applications
ELISA (1:5000); WB (1:1000)
Scientific Description
In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe2+, and oxygen.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, and store product at -20°C. Expiration date is six months from date of shipping if stored properly.
Product Specific References
1. J. Biol. Chem. 2001. 36(276): 33512-33517.  doi:10.1074/jbc.M105929200