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Glutathione Antibody, HRP

Catalog # Pack Size Price(USD)
CN1010 50 µg $250.00

Quantity:

Product Description
This antibody was developed using glutathione (GSH)-KLH conjugates and affinity purified with antigen-specific affinity chromatography. The antibody is conjugated to peroxidase (HRP) via reductive amination and could be utilized for detection and quantization of the reduced (GSH) and oxidized (GSSG) glutathione as well as glutathionylated proteins and peptides.
 
 
Western blot analysis of the glutathionylated proteins in MMRU cell lysates. Right lane: sample boiled with DTT.
Species
Rabbit
Formulation
250 µg/mL in PBS with 50% glycerol
Immunogen
Glutathione-KLH conjugates linked through -S- bonds
Conjugation
Aldehyde-activated HRP via reductive amination
HRP/Ab ratio - 3:1
Specificity
This pan-specific antibody recognizes both GSH and GSSG as well as glutathionylated proteins and peptides.
Applications
Quantitative ELISA; WB; IP (affinity chromatography); IF; IHC
Scientific Description
Glutathione is a tripeptide. It contains an unusual peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side chain. Glutathione, an antioxidant, protects cells from toxins such as free radicals. Glutathione can also modify proteins via disulfide bonds. Redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes have been reported.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Expiration date is six months from date of shipping if properly stored.

Product Specific References

1. PNAS. 2005. 102(39): 13998-14003.  doi:10.1073/pnas.0504398102
2. J. Biol. Chem. 2007. 282(45): 32640-54. doi:10.1074/jbc.M702953200