| |
|
|
|
|
 back
Anti-acetyl Lysine, HRP conjugates (ICP0381) |
| Catalog Number |
Pack Size |
Price (US) |
| ICP0381-100 |
100µg |
$350 |
|
|
|
|
|
|
|
Description: Affinity purified rabbit polyclonal anti-acetylated lysine antibody (anti-AcK) was developed using a unique technique that utilize the acetylated KLH as immunogen. The purified antibody was conjugated to horseradish peroxidase (HRP) via reductive amination. Direct label of primary antibody will avoid the use of secondary antibodies therefore eliminating the interference of the 2nd antibody-conjugates. |
|
|
|
Species: Rabbit
Formulation: 250µg/mL antibody stored in PBS, pH 7, with 50% glycerol
Storage & Stability: Store product at –200C. One year from date of shipping
Immunogen: Acetylated KLH conjugates
Purification: The antibody was purified on acetyl-lysine agarose
Conjugation: reductive amination, HRP/Ab molar ratio: 2:1
Application(s): For western blotting direct and immunohistochemistry
Specificity: Recognize proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins.
Scientific Description: Acetylation of lysine is an important reversible modification. The activity of some proteins is controlled by this process. Histone acetyltransferases (HATs) acetylate the conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) that contain lysines residues. Histone deacetylases (HDACs) removes the acetyl group from the same residue. [1] Acetylation/deacetylation of histones results in cell signaling processes that include gene activity, cell growth, differentiation and apoptosis. [2] In cancer and polyglutamine diseases, the regulation of protein acetylation/deacetylation is impaired. [3]
References: 1. Hassig, C.A. et al. Curr. Opin. Chem. Biol. 1997. 1, 3, 300-308.
2. Allfrey, V. G. et al. Proc. Natl. Acad. Sci. USA 1964, 51, 786-794.
3. Hughes, R.E. Curr. Biol. 2002. 12, R141-R143.
|
|
|
|
|
|
|
|
|
