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Description: Affinity purified rabbit polyclonal anti-p42 MAPK antibodies are developed with c-terminal peptide of human MAPK (ERK2). |
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Species: Rabbit
Formulation: 250 µg/mL in TBS, 0.1% NaN3.
Storage & Stability: Store product at –20 0C. Three years from date of shipping.
Immunogen: human p42 ERK2 c-terminal peptide-KLH conjugates.
Purification: The antibody was affinity purified with p42 ERK2 c-terminal peptide-on agarose
Specificity: The antibody recognizes p42 ERK1 of human, rat, bovine, mouse and rabbit. It does not cross-react with p44 ERK1.
Application(s): ELISA (0.5 µg/mL), WB (1 µ>g/mL), IP (4 µg/sample)
Scientific Description: Maturation or mitogen–activated protein kinase (MAPK) also known as extracellular signal regulated kinase 2 (ERK 2) is involved in eukaryotic signaling processes. [1] It is activated by a dual phosphorylation on threonine and tyrosine residue by the dual-specificity kinase MKK1/2. After stimulation, ERK2 p42 translocate to the nucleus. [2] Once phosphorylated, ERK2 can form dimers with phosphorylated and unphosphorylated ERK2 partners. ERK 2 can also phosphorylate many substrates for instance EGFR, c-PLA2, Elk1/TCF and c-Myc. [3-5]
References: 1.Cobb, M. H. et al. J. Cell Regul. 1991, 2, 965-978.
2.Chen, R. H. Mol. Cell Biol. 1992, 12, 915–927.
3.Northwood, I. C. J Biol Chem. 1991, 266, 15266–15276.
4.Lin, L. et al. Cell 1993, 72, 269–278.
5.Seth. A. et al. J Biol. Chem. 1992, 267, 24796-24804.
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mTOR (n-terminal) Antibody
