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Anti-Phospho Crosstide (ICP0190) |
| Catalog Number |
Pack Size |
Price (US) |
| ICP0190-100 |
100µg |
$285 |
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Description: Crosstide (PRAATF) is a substrate of PKB (AKT) and SGK kinase family. Affinity purified rabbit polyclonal anti-pCrosstide is developed with phosphorylated Crosstide (PRAApTF) and absorbed with non-phosphorylated Crosstide (PRAATF. The antibody has been utilized for non-radioactive PKB and SGK kinase assay. |
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Species: Rabbit
Formulation: 250 µg/mL in PBS, pH7, 0.01% NaN3.
Storage & Stability: Store product at –20 0C. Three years from date of shipping.
Immunogen: phospho-Crosstide (PRAApTF)-KLH
Purification: The antibody was affinity purified with PRAApTF on agarose follow by affinity adsorption with non-phosphorylated Crosstide
Specificity: The antibody is recognizes only the phophophorylated Crosstide, the product of SGK, PKB in a non-radioactive kinase assay. There is no cross-reaction with the non-phosphorylated Crosstide.
Conjugation: acetylation of primary amine with active ester of biotin (NHS-activated d-biotin)
Application(s): ELISA, WB, non-radioactive AKT and SGK kinase assay
Scientific Description: Crosstide is a synthetic peptide with sequence GRPRTSSFAEG). Kinases phosphorylate Crosstide to phosho-Crosstide. [1-3]
References: 1. Cross, D.A.E., et al. Nature 1995, 378, 785-789.
2. James, S.R., et al. Biochem. J. 1996, 315, 709-713.
3. Alessi, D.R., et al. EMBO J. 1996, 15, 6541-6551.
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