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Anti-phospho-threonine (Anti-pT), HRP conjugates (ICP9821)

Catalog Number Pack Size Price (US)
ICP9821-100 100µg $350


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Description: Affinity purified rabbit anti-phosphothreonine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of threonine-phosphorylated protein and peptides

Species: Rabbit

Formulation: 1 mg/mL antibody stored in PBS, pH 7, with 50% glycerol

Storage & Stability: Store product at –20 0C. One year from date of shipping.

Immunogen: Phosphothreonine-KLH conjugates and Phosvitin

Purification: The antibody was affinity purified with phosphothreonine on agarose followed by affinity adsorption with threonine on agarose.

Conjugation: Reductive amination with 2/1 molar ratio of HRP/Ab.

Specificity: The antibody is pan-specific against phosphothreonine, slightly cross-react to phosphoserine but does not cross-react with phosphotyrosine.

Application(s): ELISA (1:1000), western blot (1: 500)

Scientific Description: Threonine is one of the 20 naturally occurring amino acids. Kinases phosphorylate threonine to phosphothreonine which enters various biosynthetic pathways in the body.

References: M.J. Betts, R.B. Russell. Bioinformatics for Geneticists, M.R. Barnes, I.C. Gray eds, Wiley, 2003.

 

 
 

 

 
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