| |
|
|
|
|
 back
Anti-Methyl (mono-, di-) Lysine (ICP0501) |
| Catalog Number |
Pack Size |
Price (US) |
| ICP0501-100 |
100µg |
$250 |
|
|
|
|
|
|
|
Description: Affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a unique technique of ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues |
|
|
|
Species: Rabbit
Formulation: 250 µg/mL in PBS with 50% glycerol.
Storage & Stability: Store product at 20 0C. One year from date of shipping.
Immunogen: Methylated KLH conjugates.
Purification: The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose.
Specificity: The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methylysine). There is no cross-reaction with acetylated protein. The cross-reactivity with tri-methyllysine has not been tested.
Conjugation: Reductive amination
Application(s): ELISA, western blot, immunohistochemistry.
Scientific Description: Methylation on the lysine residue of protein (MeK) is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins for example calmodulin, cytochrome C, chromosomal proteins, histones, non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3]
References: 1. Im, H. et al. J. Biol. Chem. 2003, 278, 18346-18352.
2. Ng, H. H. et al. J. Biol. Chem. 2002, 277, 34655-34657.
3. Zegerman, P. et al. J. Biol. Chem. 2002, 277, 11621-11624.
Western blot : Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine (anti-MeK, ICP0501). Histones were isolated with cold 0.1N HCl extraction followed by method.
A: blot with 0.5 ug/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 ug/mL).
B: blot with 0.5 ug.mL of anti-MeK (ICP0501) in TBSt.
:
|
|
|
|
|
|
|
|
|
