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Anti-p70 S6K, HRP conjugates (ICP9917) |
| Catalog Number |
Pack Size |
Price (US) |
| ICP9917-100 |
100µg |
$285 |
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Description: Affinity purified rabbit polyclonal anti-p70 S6K antibodies were developed with c-terminal peptide of human S6K (p70) and were conjugated to horse-radish peroxidase through reductive amination. This conjugates has been used for one-step immuno-
blotting of crude cell lysates and immunoprecipitated p70 S6K. The use of HRP conjugates, reduce time of blotting and background, particularly the interfere of IgG bands resulting from IP.
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Western blot: One-step immunoblotting with cross-antibodies of the immunoprecipitated antibodies A and C: IP with anti-p90 RSK1
B and D: IP with anti-70 S6K
A and B: IB with anti-p70 S6K HRP, ICP 9917
C and D: IB with anti-RSK1 HRP conjugate, ICP9920
Species: Rabbit
Formulation: 250 µg/mL in PBS and 50% glycerol.
Storage & Stability: Store product at –20 0C. Three years from date of shipping.
Immunogen: human p70 S6K c-terminal peptide C-ISKRPEHLRMNL KLH conjugates.
Purification: Affinity purified with C-ISKRPEHLRMNL on agarose.
Conjugation: Reductive amination with 2/1 molar ratio of HRP/antibody.
Specificity: Recognize 70 kD S6K, not cross-react with RSK1.
Application(s): ELISA, WB.
Scientific Description: p70S6K is a protein serine/threonine kinase that exists ubiquitously. [1] p70S6K affects the activities of cell cycle. p70S6K is an important regulator of cell proliferation. [2] p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well as threonine 389. Activation of p70S6K requires a phosphoinositide 3-kinase (PI3-K)-dependent signal(s).
References: 1. Ferrari, S. et al. Crit. Rev. Biochem. Mol. Biol. 1994, 29, 6, 385-413. Review.
2. Edelmann, H. M. et al. J. Biol. Chem. 1996, 271, 2, 963-971.
3. Romanelli, A. et al. Mol. Cell Biol. 1999, 19, 4, 2921-2928.
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