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Anti-trimethyl Lysine (ICP0601) |
| Catalog Number |
Pack Size |
Price (US) |
| ICP0601-100 |
100µg |
$250.00 |
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Description: This rabbit polyclonal antibody is specifically isolated and affinity purified from the anti-serum against chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection, and quantization of proteins with N-trimethylated lysine residues.
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Immunohistochemistry : Immunohistochemistry of the human tissue with ICP0601
Species: Rabbit
Formulation: 500 µg/mL in PBS, 50% glycerol.
Storage & Stability: Store product at –20 0C. Three years from date of shipping.
Immunogen: Methylated KLH conjugates
Purification: N-(epsilon) trimethyllysine on agarose and immunoaffinity adsorbed with N-(epsilon) methylated lysine and N-(epsilon)dimethylated Lysine on agarose.
Specificity: Recognize proteins with trimethylation on lysine residues (N-epsilon)). Not cross-react with acetylated proteins and mono- and dimethylated proteins
Conjugation: FITC/Ab ratio 10:1, followed by G25 filtration
Species Tested: human, rat, mouse, bovine, pig
Application(s): ELISA 1:2000, Western blot: 1:2000, Immunoprecipitation: 5 µg/mg protein sample, Immunofluorescent staining: 5 µg/mL or 1:100
Scientific Description: In the biosynthetic pathway of l-carnitine, epsilon-N-Trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha-ketoglutarate, Fe 2+, and oxygen.
References: Va, F. M. et al. J Biol Chem. 2001, 36, 276, 33512-33517.
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