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AFB1 (Aflatoxin B1) HRP
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Product Description
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This product is developed by conjugating aflatoxin B1(AFB1) to horse radish peroxidase (HRP). It can be paired with anti-aflatoxin antibodies (ICP1204 and ICP1205) in direct ELISA assays.
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 Structure of the AFB1-HRP Conjugates
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Formulation
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500 µg/mL in PBS with 50% glycerol and 1% BSA, or lyophilized powder
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Conjugation
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Afaltoxin B1 methylcarboxyloxime linked to HRP via amine acylation. AFB1:HRP molar ratio=1:1.
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ELISA Titer
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OD50 ELISA titer for AFB1-HRP is approximately 0.1 µg/mL in plates immobilized with 0.5 µg/mL of monoclonal anti-AFB1. OD signal was 95% inhibited by 5 ng/mL of free AFB1.
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Applications
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ELISA: suggested concentration, 0.1-0.5 ng/mL; Gold colloid: to be optimized by users.
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Storage & Stability
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Product is stable for at least 30 days at room temperature and for one year when stored at –20ºC. Expiration date is 6 months from date of shipping if properly stored.
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