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AFM1 (Aflatoxin M1) BSA
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Product Description
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This product is developed by conjugating aflatoxin M1 (AFM1) to bovine serum albumin (BSA). It can be used as an antigen to paire with anti-aflatoxin antibodies (ICP1798) in both ELISA and gold colloid strip test.
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Figure 1. Structure of AFM1-BSA
Figure 2. Elisa titer of AFM1-BSA.
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Formulation
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2 mg/mL in PBS with 50% glycerol, or lyophilized powder
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Conjugation
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Aflatoxin M1 methylcarboxyloxime linked to BSA via amine acylation. AFM1:BSA ratio= 4-8:1
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ELISA Titer
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OD50 ELISA titer of AFM1-BSA is approximately 0.05 µg/mL.
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Applications
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ELISA: suggested immobilized concentration, 0.05-0.2 µg/mL; Gold colloid: to be optimized by users; Immunogen for AFM1 antibody production
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at –20ºC. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if properly stored.
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