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AFM1 (Aflatoxin M1) HRP
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| Product Description |
This product is developed by cross-linking aflatoxin M1 to horseradish peroxidase (HRP). It can be used as an HRP labeled antigen to pair with anti-aflatoxin antibodies (ICP1798) in direct ELISA assays. |
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Structure of the AFM1-HRP Conjugates  Direct competitive inhibition ELISA using immobilized anti-AFM1 (ICP1798, 0.2 ug/uL) and AFM1-HRP (ICP1788, 0.1 µg/mL), 50% inhibition occurred at 0.5-1 ng/mL of free AFM1 in a non-optimized conditions
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| Formulation |
Lyophilized powder
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Conjugation |
AFM1 methylcarboxyloxime linked to HRP via amine acylation. AFB1:HRP molar ratio=1:1 |
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| ELISA Titer |
OD50 ELISA titer for AFM1-HRP is approximately 0.05-0.1 ug/mL in plate immobilized with 0.25 ug /mL of rabbit anti-AFM1 (ICP1798). OD signal was 50% inhibited by 1 ng/mL of free AFM1. |
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Applications |
Direct ELISA : suggested concentration, 0.1-0.25 µg/mL
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| Storage & Stability |
Product is stable for at least 30 days at room temperature. For extended storage, store at –20ºC. Expire date is six months from the date of shipping if stored properly. |
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