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AFM1 (Aflatoxin M1) HRP

Catalog # Pack Size Price(USD)
ICP1788 100µg $350.00
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Product Description

This product is developed by cross-linking aflatoxin M1 to horseradish peroxidase (HRP). It can be used as an HRP labeled antigen to pair with anti-aflatoxin antibodies (ICP1798) in direct ELISA assays. 

                        
 

Structure of the AFM1-HRP Conjugates

 
 
Direct competitive inhibition ELISA using immobilized anti-AFM1 (ICP1798, 0.2 ug/uL) and AFM1-HRP (ICP1788, 0.1 µg/mL), 50% inhibition occurred at 0.5-1 ng/mL of free AFM1 in a non-optimized conditions
Formulation

Lyophilized powder

 

Conjugation

AFM1 methylcarboxyloxime linked to HRP via amine acylation. AFB1:HRP molar ratio=1:1

ELISA Titer

OD50 ELISA titer for AFM1-HRP is approximately 0.05-0.1 ug/mL in plate immobilized with 0.25 ug /mL of rabbit anti-AFM1 (ICP1798). OD signal was 50% inhibited by 1 ng/mL of free AFM1.

Applications

Direct ELISA : suggested concentration, 0.1-0.25 µg/mL

 

Storage & Stability
Product is stable for at least 30 days at room temperature. For extended storage, store at –20ºC. Expire date is six months from the date of shipping if stored properly.