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Biotin Antibody Agarose

Catalog # Pack Size Price(USD)
ICP0615 10 mg $795.00

Quantity:

Product Description
The biotin antibody is developed using biotin-KLH conjugate and purified with biotin-agarose affinity chromatography. The purified biotin antibody is chemically linked to beaded agarose through a stable covalent linkage. The biotin antibody-agarose can be utilized for reversible immunoprecipitation and affinity purification of biotinylated proteins/peptides. 
Formulation
1 mL beads in 2 mL slurry with 50% glycerol
Antibody Immobilized
10 mg/mL agarose
Applications
 IP; Immunoaffinity chromatography; 
Scientific Description
Biotin is a vitamin, which binds strongly and irreversibly to avidin. Biotin is commonly used for labelling reagents. Anti-biotin antibody binds to biotin strongly but reversibly, the binding of the biotinylated species could be competitively eluted with free biotin.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. Shake throughly before use. Expiration date is one year from date of shipping if properly stored. 
 
 
Product Specific References 
  1. Nature Methods. 2017. 14 (12): 1167-1170. 10.1038/nmeth.4465

  2. EMBO reports. 2019. 20 (1): 1-21. https://doi.org/10.15252/embr.201847192

  3. Biotechniques. 2019. doi.org/10.2144/btn-2019-0088 

  4. Science Signalling. 2020. 13(627). doi: 10.1126/scisignal.aba4200

  5. Nature Com., 2020 11(1), 3133. 10.1038/s41467-020-16929-8

  6. Cell Chemical Biology. 2021. S2451-9456(21)00442-6. doi: 10.1016/j.chembiol.2021.10.008

  7. Developmental Cell. 2021. 56(1):52-66.e7. doi: 10.1016/j.devcel.2020.12.014

  8. bioRxiv. 2021. 2021.06.17.448349. www.biorxiv.org/content/10.1101/2021.06.17.448349v1