The antibody is developed using polyhistidine peptide (HHHHHH)-KLH conjugate, and affinity-purified with HHHHHH peptide-agarose. The purified antibody is conjugated to horseradish peroxidase (HRP). The product is useful for detection and localization of His-tag proteins.

Western-blot of cell lysate with His-tag antibody HRP (ICP0851). The His-tag protein (50 kDa) in the cell lysate was enriched by IP with anti-His-tag Agarose (ICP1310).                                                       1:  Molecular marker                                     2: 10 µL of original cell lysate                         3: 10 µL of cell lysate after IP                       4: 10 µL of enriched His-tag protein 

Immunohistochemistry analysis of intact CHO cells (A) and CHO cells expressing a Hig-tag protein (B) 

The cells were fixed with 4% paraformaldehyde for 15 minutes, followed by incubation with 5 ug/mL of His-tag Antibody, HRP (ICP0851) for 1 hour at room temperature.

250 µg/mL in PBS, 50% glycerol

ELISA (1:2500); WB (0.25-1.0 µg/mL); IHC (5 µg/mL)

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.