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Dimethyl (sym) Arginine Antibody
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Product Description
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This antibody is raised from rabbits immunized with NG,NG′-dimethyl-L-arginine-KLH, and affinity purified using the NG,NG′-dimethyl-L-arginine-agarose column. The antibody is useful for detection, isolation and localization of proteins/peptides with symmetric dimethylated arginine residues.
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 Western blot of human MMRU cells with Rabbit anti-Dimethyl (sym) Arginine (ICP0811) and anti-actin antibody
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Species
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Rabbit
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Formulation
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250 µg/mL in PBS, 50% glycerol
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Immunogen
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NG,NG′-dimethyl-L-arginine-KLH
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Purification
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Affinity purified with NG,NG′-dimethyl-L-arginine-agarose and absorbed with NG-monomethyl arginine-agarose
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Specificity
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Specific to symmetric dimethylated arginine residues. No cross-reaction with aceylated or monomethylated proteins/peptides.
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Applications
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ELISA (1:2000), WB (1:1000), IP (5 µg/mg protein sample), IF (5 µg/mL), IHC (5 µg/mL)
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Scientific Description
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Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.
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References |
1. J. Neurosci. 2015. 35(37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015 |
