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Dimethyl (sym) Arginine Antibody

Catalog # Pack Size Price(USD)
ICP0811 100 μg $325.00

Quantity:

 

Product Description

 

This antibody is raised from rabbits immunized with NG,NG-dimethyl-L-arginine-KLH, and affinity purified using the NG,NG′-dimethyl-L-arginine-agarose column. The antibody is useful for detection, isolation and localization of proteins/peptides with symmetric dimethylated arginine residues.

 
 
 




















 

Western blot of human MMRU cells with Rabbit anti-Dimethyl (sym) Arginine (ICP0811) and anti-actin antibody


Immunogen Structure

 
 

 

 
 
 

 


 

Species
Rabbit  
Formulation

250 µg/mL in PBS, 50% glycerol   
Immunogen

NG,NG′-dimethyl-L-arginine-KLH 
Purification

Affinity purified with NG,NG-dimethyl-L-arginine-agarose and absorbed with NG-monomethyl arginine-agarose
Specificity
Specific to symmetric dimethylated arginine residues. No cross-reaction with aceylated or monomethylated proteins/peptides. 
Applications
ELISA (1:2000), WB (1:1000), IP (5 µg/mg protein sample), IF (5 µg/mL), IHC (5 µg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified in such a way possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.  
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.

References

1. J. Neurosci. 2015. 35(37): 12890-12902. doi: 10.1523/JNEUROSCI.0246-15.2015